| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-09-18 |
| タイトル |
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|
タイトル |
Involvement of a negative glucocorticoid response element on corticotropin-releasing factor gene promoter in hypothalamic cells |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cyclic AMP |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
corticotropin-releasing factor receptor |
| キーワード |
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主題Scheme |
Other |
|
主題 |
glucocorticoids |
| キーワード |
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主題Scheme |
Other |
|
主題 |
hypothalamus |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
promoter |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kageyama, Kazunori
Hanada, Komaki
Iwasaki, Yasumasa
Sakihara, Satoru
Nigawara, Takeshi
Kasckow, John
Suda, Toshihiro
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| 著者所属 |
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|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University |
| 著者所属 |
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|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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|
値 |
Department of Psychiatry, University of Cincinnati School of Medicine |
| 著者所属 |
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|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Corticotropin-releasing factor （CRF） plays a central role in controlling the hypothalamic-pituitary-adrenalaxis during stressful periods. CRF, produced in the hypothalamic paraventricular nucleus （PVN） in response tostress, stimulates adrenocorticotropic hormone （ACTH） release in the anterior pituitary. ACTH then stimulatesglucocorticoid release from the adrenal glands. Glucocorticoid in turn inhibits hypothalamic PVN production of CRFand pituitary production of ACTH. We previously demonstrated that protein kinase A（ PKA） pathway takes a mainpart in producing CRF in the hypothalamus. A functional cAMP-response element（ CRE） on the 5’-promoter region isimportant to increase CRF gene expression. Glucocorticoids inhibit CRF promoter activity in the pituitary cells, whilein the placenta and the bed nucleus of the stria terminalis, glucocorticoids stimulate it. The eff ect of glucocorticoids onCRF gene, therefore, would be tissue specifi c. Although to study the mechanism regulating CRF transcription in thePVN has been the lack of availability of a representative cell line, a rat fetal hypothalamic cell line has been recentlyavailable. We found that forskolin-stimulated CRF gene transcription is mediated by CRE on the CRF 5’-promoterregion in the hypothalamic cells. Both PKA and, in part, p38 mitogen-activated protein kinase pathways contribute tothe forskolin-induced CRF promoter activity. Glucocorticoid-dependent repression of forskolin-stimulated CRF promoteractivity was localized to promoter sequences between －278 and －249 bp. Taken together, these fi ndings indicate thata negative glucocorticoid regulatory element is critical for the repression of CRF gene in the hypothalamic cells. |
| 引用 |
|
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内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 59(Suppl.), 2007, p.S62-S66 |
| 書誌情報 |
弘前医学
巻 59,
号 Supplement,
p. S62-S66,
発行日 2007-11-29
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0439-1721 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
491.4 |
| NIIサブジェクト |
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|
主題Scheme |
Other |
|
主題 |
基礎医学 |
| 出版者 |
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|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_59_S62.pdf (218.6 kB)
