| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-09-18 |
| タイトル |
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|
タイトル |
Leptin can suppress the transcription of corticotropin-releasing factor in the immortalized hypothalamic neuron |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
corticotrophin-releasing factor (CRF) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
leptin |
| キーワード |
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主題Scheme |
Other |
|
主題 |
immortalized hypothalamic neuron |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Sakihara, Satoru
Kageyama, Kazunori
Nigawara, Takeshi
Hanada, Komaki
Suda, Toshihiro
|
| 著者所属 |
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|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Corticotropin-releasing factor （CRF） is primary regulator of hypothalamus-pituitary-adrenal（ HPA） axis,which is activated by various types of stress. Starvation is also a potent activating factor of CRF. Because theperipheral energy store refl ects on plasma levels of leptin, secreted from adipose tissue, it is presumed that leptin maybe involved in the CRF activity during starvation. Indeed, the attenuation of CRF or HPA axis activity by leptintreatment has been demonstrated in several reports. While many studies indicate the leptins involvement in CRFactivity, its precise regulation has not been elucidated. The aim of this study is to determine the impact of leptin onthe hypothalamic CRF neuron. To exclude the other hypothalamic factors, associated with leptins central eff ect, weemployed the in vitro study using immortalized CRF expressing neuron（ IVB cell）. Since the mRNA expression of longformleptin receptor was detected clearly in IVB cell with RT-PCR, we next investigated the impact of leptin on this cell.IVB cell was treated with leptin in diff erent concentrations and periods, then, the mRNA expression and the promoteractivity of CRF were analyzed with real-time PCR and luciferase assay, respectively. In result, leptin suppressed CRFpromoter activity with dose- and time- dependent manner in IVB cell. This indicates that leptin can suppress the CRFtranscription directly in hypothalamus. Our result might explain the mechanism partially how leptin attenuate theactivation of CRF or HPA axis induced by stress or starvation. |
| 引用 |
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内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 59(Suppl.), 2007, p.S193-S198 |
| 書誌情報 |
弘前医学
巻 59,
号 Supplement,
p. S193-S198,
発行日 2007-11-29
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0439-1721 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
491 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
基礎医学 |
| 出版者 |
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出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
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|
値 |
Article |
HirosakiMedJ_59_S193.pdf (488.2 kB)
