| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-09-18 |
| タイトル |
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|
タイトル |
Protective eff ect of intranasal vaccination with nontoxic mutant TSST-1 against Staphylococcus aureus infection |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Narita, Koji
Hu, Dong-Liang
Tsuji, Takao
Nakane, Akio
|
| 著者所属 |
|
|
値 |
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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|
値 |
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Microbiology, Fujita Health University, School of Medicine, Toyoake |
| 著者所属 |
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|
値 |
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Infection caused by methicillin-resistant Staphylococcus aureus （MRSA） has been the most commonlyacquired types of nosocomial infections. It was reported that anterior nares are the major reservoir of S. aureus andthe source of 80% of S. aureus bacteremia is endogenous. Considering these facts, elimination and reduction of nasalcarriage are thought to be eff ective protection against systemic S. aureus infection and nosocomial infection. Toxic shocksyndrome toxin 1（ TSST-1） is one of superantigens secreted by S. aureus. Previously, it was reported that mutant form（H135A） of TSST-1（ mTSST-1） was shown to be nontoxic, and subcutaneous vaccination with mTSST-1 could protectagainst systemic S. aureus infection in a mouse model. In this study, we investigated the protective eff ect of intranasalvaccination with mTSST-1 supplemented with non-toxic mutant（ H44A） Escherichia coli heat labile toxin（ mLT） as amucosal adjuvant. The results demonstrated that intranasal immunization with mTSST-1 plus mLT could efficientlyinduce production of anti-TSST-1 antibodies in sera and also induce anti-TSST-1 IgA production in bronchoalveolar lavagefl uids（ BALF） of vaccinated mice. In nasal-associated lymphoid tissues（ NALT） of vaccinated mice, anti-TSST-1 IgAsecreting cells were signifi cantly increased. To evaluate of the protective eff ect of this vaccine against systemic S. aureusinfection, BALB/c mice were vaccinated with mTSST-1 plus mLT and challenged with clinical isolated S. aureus 834intravenously. Bacterial numbers in spleen and liver, and cumulative mortality rate of vaccinated mice were lower thanthose of control mice. We further developed a mouse model of nasal S. aureus colonization. S. aureus bacterial numbers innasal cavity of vaccinated mice were signifi cantly reduced compared with those of control mice. These results indicatethat intranasal immunization with mTSST-1 plus mLT is able to induce systemic and mucous immune responses and ofprovide protection against systemic S. aureus infection and nasal colonization. |
| 引用 |
|
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内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 59(Suppl.), 2007, p.S227-S234 |
| 書誌情報 |
弘前医学
巻 59,
号 Supplement,
p. S227-S234,
発行日 2007-11-29
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0439-1721 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
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|
主題Scheme |
NDC |
|
主題 |
491 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
基礎医学 |
| 出版者 |
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出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
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|
値 |
Article |
HirosakiMedJ_59_S227.pdf (275.9 kB)
