| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-08-19 |
| タイトル |
|
|
タイトル |
＜Poster＞Neurovascular coupling studies in awake-behaving mice |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
laser-Doppler flowmetry |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Functional brain imaging |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
whisker stimulation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
behavioral analysis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Takuwa, Hiroyuki
Masamoto, Kazuto
Obata, Takayuki
Kanno, Iwao
|
| 著者所属 |
|
|
値 |
National Institute of Radiological Science |
| 著者所属 |
|
|
値 |
National Institute of Radiological Science |
| 著者所属 |
|
|
値 |
National Institute of Radiological Science |
| 著者所属 |
|
|
値 |
National Institute of Radiological Science |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The mechanism regulating cerebral blood fl ow （CBF） during brain function （neurovascular coupling）has been widely investigated in animals under anesthetized conditions, though anesthesia is known to greatly aff ect neurovascular physiology. The present study aims to develop a novel model for neurovascular coupling studies in awake-behaving mice. Male C57BL/6J mice were initially anesthetized with isofl urane in preparation for attaching the study apparatus to the head. The animal was tethered to the study apparatus but allowed to move spontaneously on a floated ball. The animal behavior and regional CBF in the somatosensory barrel cortex were simultaneously measured with optical motion sensor and laser-Doppler fl owmetry （LDF）, respectively. Anesthesia was discontinued during recovery, while whisker stimulation （frequency 10 Hz and duration 10 or 20 sec） was induced at either the contralateral or ipsilateral side of the LDF recording site. During the experiments, the animals showed no signs of struggling against the head restraint. The intensity of baseline CBF was higher while the animal was under 2% isoflurane aesthesia than it was after anesthesia was stopped. CBF response to stimulation was not observed under anesthesia. After the animal was recovered from anesthesia, an increase in CBF （34 ± 18%） was observed during contralateral stimulation but not during ipsilateral stimulation. The fl uctuation levels of CBF baseline during resting and walking conditions were ±2.7% and ±3.5%, respectively. We observed that these fl uctuations were not due to vibration noises caused by such as air-puff and animal motion in our experimental conditions. |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 61(Suppl.), 2010, p.S192-S196 |
| 書誌情報 |
弘前医学
巻 61,
号 Supplement,
p. S192-S196,
発行日 2010-07-08
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_61_S192.pdf (135.9 kB)
