| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-04-30 |
| タイトル |
|
|
タイトル |
Differentiation Inhibition of Regulatory T Cells in Hirosaki Hairless Rat Due to Deletion of The LY49 Family Genes Expressed in Thymic T Cells and Dendritic Cells |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
regulatory T cell |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
the Ly49 family genes |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hirosaki hairless rat |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Yamada, Toshiyuki
Nanashima, Naoki
Shimizu, Takeshi
Tsuchida, Shigeki
|
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11952 |
|
|
姓名 |
ヤマダ, トシユキ |
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11951 |
|
|
姓名 |
ナナシマ, ナオキ |
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11954 |
|
|
姓名 |
シミズ, タケシ |
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11328 |
|
|
姓名 |
ツチダ, シゲキ |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11958 |
|
|
姓名 |
山田, 俊幸 |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11957 |
|
|
識別子Scheme |
研究者総覧 |
|
|
識別子URI |
http://hue2.jm.hirosaki-u.ac.jp/html/416_ja.html |
|
|
識別子 |
416 |
|
|
姓名 |
七島, 直樹 |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11960 |
|
|
識別子Scheme |
研究者総覧 |
|
|
識別子URI |
http://hue2.jm.hirosaki-u.ac.jp/html/200_ja.html |
|
|
識別子 |
200 |
|
|
姓名 |
清水, 武史 |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
11326 |
|
|
姓名 |
土田, 成紀 |
| 著者所属 |
|
|
値 |
Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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|
値 |
Department of Biomedical Science, Hirosaki University Graduate School of Health Science |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
he Hirosaki hairless rat （HHR） is a mutant strain derived from the Sprague-Dawley rat （SDR）. The HHR thymus was markedly small and showed underdeveloped medulla. In the HHR thymus, CD4 levels were decreased, suggesting that differentiation of CD4-positive T cells is disturbed. Because naturally occurring regulatory T （nTreg） cells complete the differentiation in the thymus, the maturation status of the cells was examined in the HHR thymus. Real-time PCR revealed reduced expression of the Foxp3 and CD25 （IL-2Rα） genes, important for nTreg differentiation, and flow cytometric analysis showed decreased number of CD4+CD25+Foxp3+nTreg cells, indicating that mature nTreg formation is repressed. To explore genes responsible for the failure of nTreg differentiation, comparative genome hybridization array was performed using DNA from HHR and SDR. This analysis identified two regions deleted on HHR chromosome 4. One region contained the Klra17 （similar to Ly49si1） gene and the other the Ly49s3, Ly49s4, Ly49i3 and Ly49i4 genes. RT-PCR of T cells and dendritic cells（ DCs）isolated from the SDR thymus revealed that the Klra17 gene was expressed in CD4-single positive （CD4-SP） cells and the Ly49s3 gene in DCs, indicating that HHR loses expression of the Klra17 and Ly49s3 genes in thymic CD4-SP cells and DCs, respectively. The mixed culture of CD4-SP cells and DCs from the HHR thymus exhibited poor nTreg differentiation. Taken together, these results suggest that the Klra17 and Ly49s3 genes are responsible for the differentiation of nTreg cells. |
| 引用 |
|
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内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 64(Suppl.), 2013, p.S65-S69 |
| 書誌情報 |
弘前医学
巻 64,
号 Supplement,
p. S65-S69,
発行日 2013-04-02
|
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_64_S65.pdf (393.4 kB)
