| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2016-03-31 |
| タイトル |
|
|
タイトル |
TGF-β1 Induces Fibroblast Proliferation Partly Through Actibation of RaF/MEK/ERK Signaling Pathway by Inhibition of RAF-KINASE Inhibitor Protein Expression |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Raf-kinase inhibitor protein |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
transforming growth factor β |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
fibroblast |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
proliferation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
signal transduction |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kambe, Yuki
Yamazaki, Takehiko
Nakano, Hajime
Hanada, Katsumi
Tamai, Katsuto
Tsuchida, Shigeki
Sawamura, Daisuke
|
| 著者所属 |
|
|
|
Departments of Dermatology Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Departments of Dermatology Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Departments of Dermatology Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Departments of Dermatology Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Departments of Dermatology Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Departments of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Departments of Dermatology Hirosaki University Graduate School of Medicine |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The Raf-kinase inhibitor protein （RKIP） physically binds to Raf and MEK, and inhibits the Raf/MEK/ERK signaling pathway. Transforming growth factor （TGF）-β1 is a multifunctional cytokine that has a proliferative effect on fibroblasts. A considerable effort has been applied to studies of cell growth inhibition by TGF-β1, whereas far less attention has been given to the elucidation of the mechanism of TGF-β1-induced growth stimulation. In this study, we revealed that TGF-β1 significantly enhanced the proliferation of normal human dermal fibroblasts（ NHDFs）. In addition, we showed that TGF-β1 inhibited the expression of RKIP mRNA and protein. Moreover, a transfection experiment demonstrated that overexpression of RKIP significantly inhibited NHDF cell growth. These data point to a relationship between enhanced NHDF cell growth and reduction of RKIP expression by TGF-β1, suggesting that TGF-β1 may induce fibroblast proliferation partly through the inhibition of RKIP. Furthermore, we revealed that EGF and PDGF-BB, known activators of the Raf/MEK/ERK signal transduction pathway, failed to modulate RKIP
expression. Finally, TGF-β1 induced a transient phosphorylation of ERK1/2. Although detailed mechanisms of NHDF proliferation via RKIP are yet unknown, it will be important to investigate the molecular status of RKIP in certain proliferative diseases, such as keloids, in which TGF-β1 has been reported to play a pathogenic role by inducing hyperproliferation of dermal fibroblasts. |
| 引用 |
|
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内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 66, 2016, p.120-126 |
| 書誌情報 |
弘前医学
巻 66,
号 2-4,
p. 120-126,
発行日 2016-03-25
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_66(2-4)_120.pdf (733.4 kB)
