| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2016-03-31 |
| タイトル |
|
|
タイトル |
In Vivo Functional Adaptive Immune Responses in Mice Subjected to Allogeneic Umbilical Cord Blood Cell Transplantation |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
umbilical cord blood cell |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
allogeneic transplantation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
hematopoietic stem cell |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
adaptive immune system |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
murine model |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Maeda, Hiroshi
Sato, Hideaki
Araya, Mika
Ito, Kyoko
Terui, Ken-ichiro
Tsushima, Eiki
Nakano, Manabu
Ito, Koichi
|
| 著者所属 |
|
|
値 |
Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences |
| 著者所属 |
|
|
値 |
Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, StemCell Institute Inc. |
| 著者所属 |
|
|
値 |
Department of Medical Technology, Hirosaki University School of Health Sciences |
| 著者所属 |
|
|
値 |
Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences |
| 著者所属 |
|
|
値 |
Department of Cellular Biochemistry and Molecular Biology, Hirosaki University Graduate School of Health Science |
| 著者所属 |
|
|
値 |
Department of Development and Aging, Hirosaki University School of Health Sciences |
| 著者所属 |
|
|
値 |
Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences |
| 著者所属 |
|
|
値 |
Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Using a murine model, we investigated whether allogeneic umbilical cord blood cell （UCBC） transplantation facilitates immune reconstitution with functional maturity in comparison with bone marrow cell （BMC） transplantation. UCBCs and BMCs prepared from green fluorescent protein （GFP）-transgenic C57BL/6 （H-2b） mice were transferred into BALB/c （H-2d） mice that had been subjected to lethal total body X-ray irradiation. Although UCBC-transplanted mice showed poorer survival than BMC-transplanted mice for the same number of transplanted cells, increasing the number of transplanted UCBCs improved survival with an increase of donorderived GFP+ cell engraftment. Flow cytometric analysis revealed development of GFP+ immune cells of donor origin, including T cells, B cells, monocytes, and granulocytes, in the peripheral blood of both sets of recipient mice. Furthermore, the functional maturity of T and B cells involved in adaptive immunity following allogeneic UCBCand BMC-transplantation was assessed in T- and B-deficient RAG2-/-BALB/c mice. Both sets of recipients showed complete rejection of third-party C3H （H-2k） skin grafts and antibody responses to the T cell-dependent antigen, 2,4,6-trinitrophenyl-keyhole limpet hemocyanin, with immunoglobulin class switching. These results suggest that UCBCs have essentially the same ability as BMCs to reconstitute a functional adaptive immune system, even in an allogeneic environment. |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 66, 2016, p.162-175 |
| 書誌情報 |
弘前医学
巻 66,
号 2-4,
p. 162-175,
発行日 2016-03-25
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_66(2-4)_162.pdf (1.0 MB)
