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Fatty acid synthase-positive hepatocytes and subsequent steatosis in rat livers by irinotecan
http://hdl.handle.net/10129/5663
http://hdl.handle.net/10129/566352a4f824-d7e3-4a43-9930-299577dcee7d
| 名前 / ファイル | ライセンス | アクション |
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本文 (759.2 kB)
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審査要旨 (129.7 kB)
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内容要旨 (177.5 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2015-10-27 | |||||||
| タイトル | ||||||||
| タイトル | Fatty acid synthase-positive hepatocytes and subsequent steatosis in rat livers by irinotecan | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | fatty acid synthase | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | liver progenitor cell | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Kupffer cell | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | chemotherapy-associated steatohepatitis | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | irinotecan | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
Sawano, Takeyuki
× Sawano, Takeyuki
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| 著者(ヨミ) | ||||||||
| 識別子Scheme | WEKO | |||||||
| 識別子 | 16030 | |||||||
| 姓名 | サワノ, タケユキ | |||||||
| 著者別名 | ||||||||
| 識別子Scheme | WEKO | |||||||
| 識別子 | 16031 | |||||||
| 姓名 | 澤野, 武行 | |||||||
| 著者所属 | ||||||||
| 値 | 弘前大学大学院医学研究科 | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Using a rat model, I investigated factors contributing to the pathogenesis of irinotecan-associated fatty liver disease. Male Sprague-Dawley rats were administered 200 mg/kg irinotecan by intraperitoneal injection on days 1-4, but not on days 5-7. This schedule was repeated 3 times. Rats were sacrificed 4, 18 and 25 days after the last injection, and liver steatosis was evaluated by hematoxylin and eosin (H&E) staining, microarray analysis and immunohistochemistry. Panacinar intrahepatocyte vacuoles were absent on days 4 and 25, but present on day 18, and this alteration was more prominent around the bile ducts than the central veins. Microarray analysis showed that the expression of genes involved in the synthesis of cholesterol and fatty acids was upregulated on day 4. Immunohistochemistry detected fatty acid synthase (Fasn)-strongly positive hepatocytes as well as the activation of liver progenitor cells on day 4, whereas intracellular vacuoles were evident in carbonic anhydrase 3 (CA3)-positive hepatocytes on day 18. Thus, irinotecan-induced liver steatosis was preceded by Fasn-strongly-positive hepatocytes and liver progenitor cell activation. The magnitude of the decrease in the number of Fasn-strongly positive hepatocytes between days 4 and 18 was similar to that of the increase in the number of CA3-positive hepatocytes accompanying vacuoles. | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 掲載誌 Oncology reports, 33(5), p.2151-2160, 2015 https://www.spandidos-publications.com/or/33/5/2151 DOI: 10.3892/or.2015.3814 |
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| 書誌情報 | p. 1-33, 発行日 2015-09-30 | |||||||
| 日本十進分類法 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 491.6 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | ETD | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | Irinotecan投与によるラット肝の脂肪化誘発とそれに先行する脂肪酸合成酵素陽性細胞の出現 | |||||||
| タイトル（ヨミ） | ||||||||
| その他のタイトル | Irinotecan トウヨ ニヨル ラットカン ノ シボウカ ユウハツ ト ソレニ センコウ スル シボウサン ゴウセイ コウソ ヨウセイ サイボウ ノ シュツゲン | |||||||
| NIIサブジェクト | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | 医学 | |||||||
| 資源タイプ | ||||||||
| 値 | Thesis | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関名 | 弘前大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2015-09-30 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲1902 | |||||||
