| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-03-08 |
| タイトル |
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タイトル |
Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Fujita, Toshitsugu
Kitaura, Fusako
Yuno, Miyuki
Suzuki, Yutaka
Sugano, Sumio
Fujii, Hodaka
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| 著者所属 |
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値 |
Hirosaki Univ, Grad Sch Med, Dept Biochem & Genome Biol |
| 著者所属 |
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値 |
Osaka Univ, Res Inst Microbial Dis, Combined Program Microbiol & Immunol, Chromatin Biochem Res Grp |
| 著者所属 |
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値 |
Osaka Univ, Res Inst Microbial Dis, Combined Program Microbiol & Immunol, Chromatin Biochem Res Grp |
| 著者所属 |
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値 |
Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci |
| 著者所属 |
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値 |
Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci |
| 著者所属 |
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値 |
Hirosaki Univ, Grad Sch Med, Dept Biochem & Genome Biol |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Chromosomal interactions regulate genome functions, such as transcription, via dynamic chromosomal organization in the nucleus. In this study, we attempted to identify genomic regions that physically bind to the promoter region of the Pax5 gene, which encodes a master regulator for B cell lineage commitment, in a chicken B cell line, DT40, with the goal of obtaining mechanistic insight into transcriptional regulation through chromosomal interaction. We found that the Pax5 promoter bound to multiple genomic regions using locus-specific chromatin immunoprecipitation (locus-specific ChIP), a method for locus-specific isolation of target genomic regions, in combination with next-generation sequencing (NGS). Comparing chromosomal interactions in wild-type DT40 with those in a macrophage-like counterpart, we found that some of the identified chromosomal interactions were organized in a B cell-specific manner. In addition, deletion of a B cell-specific interacting genomic region in chromosome 11, which was marked by active enhancer histone modifications, resulted in moderate but significant down-regulation of Pax5 transcription. Together, these results suggested that Pax5 transcription in DT40 is regulated by B cell-specific inter-chromosomal interactions. Moreover, these analyses showed that locus-specific ChIP combined with NGS analysis is useful for non-biased identification of functional genomic regions that physically interact with a locus of interest. |
| 書誌情報 |
DNA RESEARCH
巻 24,
号 5,
p. 537-548,
発行日 2017-10
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1340-2838 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/dnares/dsx023 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 資源タイプ |
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値 |
Article |
dsx023 (494.9 kB)
