| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-11-22 |
| タイトル |
|
|
タイトル |
Effect of low-concentration amyloid-β 1–42 (Aβ42) on human neuroblastoma SH-SY5Y cell viability: neuroprotective potential of combination use with carnosic acid, rebamipide, edaravone, and resveratrol |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
low-concentration Aβ42 |
| キーワード |
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主題Scheme |
Other |
|
主題 |
cell viability |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
combination treatment |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
SH-SY5Y cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Alzheimer’s disease |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Yoshida, Hidemi
Hashimoto, Yuko
Fukushima, Takashi
Tanji, Kunikazu
Matsumiya, Tomoh
Seya, Kazuhiko
Kawaguchi, Shogo
Imaizumi, Tadaatsu
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| 著者所属 |
|
|
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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Department of Neuropathology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Vascular Biology Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Toxic amyloid-beta （Aβ） is known to generate symptoms of Alzheimer’s disease （AD）; however, less is known regarding the neurotoxicity of Aβ at lower concentrations. Moreover, the neuroprotective potential of combination treatment with plant biophenols and existing drugs is not well understood. In this study, we estimated the no-observed adverse effect level （NOAEL） of Aβ 1–42 （Aβ42） against cultured human neuroblastoma SHSY5Y cells, and examined the neuroprotective effect of combination pretreatment with 10 μM carnosic acid, 30 nM rebamipide, 10 μM edaravone, and 10 μM of resveratrol （the “CRER” blend） on weak but toxic Aβ42-treated SH-SY5Y cells. We evaluated the NOAEL of Aβ42 at 500 nM in these cells. Aβ42 at 1–8 μM reduced cell viability; however, the “CRER” blend ameliorated this Aβ42-induced decrease in viability. The “CRER” blend induced the expression of Aβ-degrading enzymes including matrix metalloproteinase-14 （MMP-14） and neprilysin, while also enhancing the expression of the inducible α-secretase TACE （tumor necrosis factor-α-converting enzyme）, sirtuin 1 （SIRT1）, and brain-derived neurotrophic factor（ BDNF）. Collectively, our results indicate that the “CRER” may aid in the prevention of Aβ toxicity by enhancing MMP-14, neprilysin, TACE, SIRT1, and BDNF. Thus, the “CRER” blend may prove to be a promising strategy for the prevention of Aβ-mediated disorders, particularly AD. |
| 書誌情報 |
ja : 弘前医学
巻 70,
号 1,
p. 24-38,
発行日 2019-11-01
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| ISSN |
|
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0439-1721 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
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|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
HirosakiMedJ_70(1)_24 (420.2 kB)
