| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2020-11-12 |
| タイトル |
|
|
タイトル |
Neuronal differentiation potential of mouse mesenchymal stem cells derived from white versus brown adipose tissue |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Autologous |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Brown adipose tissue |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Mesenchymal stromal cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Neuronal differentiation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
White adipose tissue |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Nitobe, Yohshiro
Kumagai, Gentaro
Sasaki, Ayako
Nagaoki, Toshihide
Wada, Kanichiro
Tanaka, Sunao
Asari, Toru
Furukawa, Ken-Ichi
Ishibashi, Yasuyuki
|
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
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Department of Pharmacology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
|
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Mesenchymal stem cells（ MSCs） from white adipose tissue（ WAT） have a higher osteogenic differentiation potential than those from brown adipose tissue （BAT）. The purpose of this study was to compare the neuronal differentiation potentials of these cells. In addition, to examine the applicability of these cells for autologous neuronal regeneration, the mouse hind limbs were assessed for neurological function after adipose tissue was harvested from the inguinal and interscapular regions. Adipose-MSCs were isolated from WAT of the inguinal subcutaneous regions and BAT of the interscapular regions in 6-week-old female C57BL/6J mice. The expression of cell-surface markers on the adipose-MSCs was examined by flowcytometry. The markers examined were CD90, Sca1, CD34, and CD45. Neuronal differentiation was assessed by immunocytochemistry using NeuN as a mature neuron marker. MSCs derived from WAT and BAT displayed similar immunophenotypes. Both cell populations differentiated into NeuNpositive cells（ 16.2% ± 1.0% of the WAT-MSCs and 14.7% ± 2.4% of the BAT-MSCs） in vitro. Harvesting the adipose tissue did not affect the neurological properties of the hind limbs. These findings indicate that both WAT-MSCs and BAT-MSCs may be attractive cells for autologous euronalregeneration. |
| 書誌情報 |
弘前医学
巻 71,
号 1,
p. 12-20,
発行日 2020-10-30
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_71(1)_12 (332.5 kB)
