WEKO3
アイテム
{"_buckets": {"deposit": "f35f75e5-69c4-407d-873b-a784efed9ada"}, "_deposit": {"created_by": 11, "id": "7176", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "7176"}, "status": "published"}, "_oai": {"id": "oai:hirosaki.repo.nii.ac.jp:00007176", "sets": ["1115"]}, "author_link": ["14831", "11403", "11997", "11879", "19746", "11458", "2405"], "item_3_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2023-03-22", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-4", "bibliographicPageEnd": "29", "bibliographicPageStart": "20", "bibliographicVolumeNumber": "73", "bibliographic_titles": [{"bibliographic_title": "弘前医学"}, {"bibliographic_title": "Hirosaki Medical Journal", "bibliographic_titleLang": "en"}]}]}, "item_3_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objective: Wingless/integrase 5a(Wnt5a) pathway is known to regulate the osteogenesis. In this study, we aimed to clarify the role of Wnt5a pathway in aortic valve ectopic calcification.\nMethods: Human aortic valve interstitial cells(HAVICs) were obtained from calcified aortic valves of patients with calcific aortic valve stenosis(CAVS). HAVICs were separated to CD34-negative and -positive cells by flow cytometry. We measured real-time PCR, alkaline phosphatase(ALP) activity, Alizarin Red S staining as an index of calcification. Immunohistochemical staining was performed to confirm the distribution of Wnt5a on calcified and normal aortic valves.\nResults: HAVICs, especially, CD34-negative cells are highly sensitive to tumor necrosis factor-α( TNF-α, 30 ng/mL), and which accelerated the Wnt5a gene expression. Wnt5a antagonist, box5, did not down-regulate HAVIC calcification induced by TNF-α. Further, Wnt5a agonist, foxy5, did not accelerated HAVIC calcification induced by TNF-α. There was no significant difference in the TNF-α-induced acceleration of ALP activity between the cells treated with foxy5 and box5. Furthermore, the proportion of Wnt5a positive cells was no difference between calcified and normal valves.\nConclusions: We confirmed that Wnt5a pathway does not regulate the TNF-α-induced aortic valve calcification in HAVICs obtained from CAVS patients.", "subitem_description_type": "Abstract"}]}, "item_3_publisher_35": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "弘前大学大学院医学研究科"}]}, "item_3_radio_42": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_radio_item": "Article"}]}, "item_3_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00211444", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0439-1721", "subitem_source_identifier_type": "ISSN"}]}, "item_3_text_4": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"}, {"subitem_text_value": "Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"}, {"subitem_text_value": "Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"}, {"subitem_text_value": "Department of Vascular Biology, Hirosaki University Graduate School of Medicine"}, {"subitem_text_value": "Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"}, {"subitem_text_value": "Department of Pharmacology, Hirosaki University Graduate School of Medicine"}, {"subitem_text_value": "Department of Vascular Biology, Hirosaki University Graduate School of Medicine"}]}, "item_3_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yu, Zaiqiang"}], "nameIdentifiers": [{"nameIdentifier": "19746", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Daitoku, Kazuyuki"}], "nameIdentifiers": [{"nameIdentifier": "11879", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "254", "nameIdentifierScheme": "研究者総覧", "nameIdentifierURI": "http://hue2.jm.hirosaki-u.ac.jp/html/254_ja.html"}]}, {"creatorNames": [{"creatorName": "Fukuda, Ikuo"}], "nameIdentifiers": [{"nameIdentifier": "2405", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imaizumi, Tadaatsu"}], "nameIdentifiers": [{"nameIdentifier": "11458", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "318", "nameIdentifierScheme": "研究者総覧", "nameIdentifierURI": "http://hue2.jm.hirosaki-u.ac.jp/html/318_ja.html"}]}, {"creatorNames": [{"creatorName": "Minakawa, Masahito"}], "nameIdentifiers": [{"nameIdentifier": "11403", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "251", "nameIdentifierScheme": "研究者総覧", "nameIdentifierURI": "http://hue2.jm.hirosaki-u.ac.jp/html/251_ja.html"}]}, {"creatorNames": [{"creatorName": "Furukawa, Ken-Ichi"}], "nameIdentifiers": [{"nameIdentifier": "11997", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seya, Kazuhiko"}], "nameIdentifiers": [{"nameIdentifier": "14831", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "203", "nameIdentifierScheme": "研究者総覧", "nameIdentifierURI": "http://hue2.jm.hirosaki-u.ac.jp/html/203_ja.html"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-04-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Hirosaki Med J 73-1-4_20.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "Hirosaki Med J 73-1-4_20", "url": "https://hirosaki.repo.nii.ac.jp/record/7176/files/Hirosaki Med J 73-1-4_20.pdf"}, "version_id": "aa455e32-688e-4fb1-b7a3-b6b4df61fa0b"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Calcific aortic valve stenosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Aortic valve calcification", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Wingless/integrase 5A", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bone morphogenetic protein 2", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis"}]}, "item_type_id": "3", "owner": "11", "path": ["1115"], "permalink_uri": "http://hdl.handle.net/10129/00008197", "pubdate": {"attribute_name": "公開日", "attribute_value": "2023-04-21"}, "publish_date": "2023-04-21", "publish_status": "0", "recid": "7176", "relation": {}, "relation_version_is_last": true, "title": ["Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis"], "weko_shared_id": -1}
Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis
http://hdl.handle.net/10129/00008197
http://hdl.handle.net/10129/00008197f37a4476-c155-4b14-b818-435a4e1da3e1
名前 / ファイル | ライセンス | アクション |
---|---|---|
Hirosaki Med J 73-1-4_20 (1.2 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2023-04-21 | |||||
タイトル | ||||||
タイトル | Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Calcific aortic valve stenosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aortic valve calcification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Wingless/integrase 5A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone morphogenetic protein 2 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yu, Zaiqiang
× Yu, Zaiqiang× Daitoku, Kazuyuki× Fukuda, Ikuo× Imaizumi, Tadaatsu× Minakawa, Masahito× Furukawa, Ken-Ichi× Seya, Kazuhiko |
|||||
著者所属 | ||||||
Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Vascular Biology, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Vascular Biology, Hirosaki University Graduate School of Medicine | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objective: Wingless/integrase 5a(Wnt5a) pathway is known to regulate the osteogenesis. In this study, we aimed to clarify the role of Wnt5a pathway in aortic valve ectopic calcification. Methods: Human aortic valve interstitial cells(HAVICs) were obtained from calcified aortic valves of patients with calcific aortic valve stenosis(CAVS). HAVICs were separated to CD34-negative and -positive cells by flow cytometry. We measured real-time PCR, alkaline phosphatase(ALP) activity, Alizarin Red S staining as an index of calcification. Immunohistochemical staining was performed to confirm the distribution of Wnt5a on calcified and normal aortic valves. Results: HAVICs, especially, CD34-negative cells are highly sensitive to tumor necrosis factor-α( TNF-α, 30 ng/mL), and which accelerated the Wnt5a gene expression. Wnt5a antagonist, box5, did not down-regulate HAVIC calcification induced by TNF-α. Further, Wnt5a agonist, foxy5, did not accelerated HAVIC calcification induced by TNF-α. There was no significant difference in the TNF-α-induced acceleration of ALP activity between the cells treated with foxy5 and box5. Furthermore, the proportion of Wnt5a positive cells was no difference between calcified and normal valves. Conclusions: We confirmed that Wnt5a pathway does not regulate the TNF-α-induced aortic valve calcification in HAVICs obtained from CAVS patients. |
|||||
書誌情報 |
弘前医学 en : Hirosaki Medical Journal 巻 73, 号 1-4, p. 20-29, 発行日 2023-03-22 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0439-1721 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00211444 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 弘前大学大学院医学研究科 | |||||
資源タイプ | ||||||
値 | Article |