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Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis
http://hdl.handle.net/10129/00008197
http://hdl.handle.net/10129/00008197f37a4476-c155-4b14-b818-435a4e1da3e1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Hirosaki Med J 73-1-4_20 (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2023-04-21 | |||||
| タイトル | ||||||
| タイトル | Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Calcific aortic valve stenosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Aortic valve calcification | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Wingless/integrase 5A | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Bone morphogenetic protein 2 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yu, Zaiqiang
× Yu, Zaiqiang× Daitoku, Kazuyuki× Fukuda, Ikuo× Imaizumi, Tadaatsu× Minakawa, Masahito× Furukawa, Ken-Ichi× Seya, Kazuhiko |
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| 著者所属 | ||||||
| 値 | Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Vascular Biology, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pharmacology, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Vascular Biology, Hirosaki University Graduate School of Medicine | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objective: Wingless/integrase 5a（Wnt5a） pathway is known to regulate the osteogenesis. In this study, we aimed to clarify the role of Wnt5a pathway in aortic valve ectopic calcification. Methods: Human aortic valve interstitial cells（HAVICs） were obtained from calcified aortic valves of patients with calcific aortic valve stenosis（CAVS）. HAVICs were separated to CD34-negative and -positive cells by flow cytometry. We measured real-time PCR, alkaline phosphatase（ALP） activity, Alizarin Red S staining as an index of calcification. Immunohistochemical staining was performed to confirm the distribution of Wnt5a on calcified and normal aortic valves. Results: HAVICs, especially, CD34-negative cells are highly sensitive to tumor necrosis factor-α（ TNF-α, 30 ng/mL）, and which accelerated the Wnt5a gene expression. Wnt5a antagonist, box5, did not down-regulate HAVIC calcification induced by TNF-α. Further, Wnt5a agonist, foxy5, did not accelerated HAVIC calcification induced by TNF-α. There was no significant difference in the TNF-α-induced acceleration of ALP activity between the cells treated with foxy5 and box5. Furthermore, the proportion of Wnt5a positive cells was no difference between calcified and normal valves. Conclusions: We confirmed that Wnt5a pathway does not regulate the TNF-α-induced aortic valve calcification in HAVICs obtained from CAVS patients. |
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| 書誌情報 |
弘前医学 en : Hirosaki Medical Journal 巻 73, 号 1-4, p. 20-29, 発行日 2023-03-22 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0439-1721 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00211444 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 弘前大学大学院医学研究科 | |||||
| 資源タイプ | ||||||
| 値 | Article | |||||
