| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-04-21 |
| タイトル |
|
|
タイトル |
Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Calcific aortic valve stenosis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Aortic valve calcification |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Wingless/integrase 5A |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Bone morphogenetic protein 2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Yu, Zaiqiang
Daitoku, Kazuyuki
Fukuda, Ikuo
Imaizumi, Tadaatsu
Minakawa, Masahito
Furukawa, Ken-Ichi
Seya, Kazuhiko
|
| 著者所属 |
|
|
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Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
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Department of Pharmacology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
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Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Objective: Wingless/integrase 5a（Wnt5a） pathway is known to regulate the osteogenesis. In this study, we aimed to clarify the role of Wnt5a pathway in aortic valve ectopic calcification.
Methods: Human aortic valve interstitial cells（HAVICs） were obtained from calcified aortic valves of patients with calcific aortic valve stenosis（CAVS）. HAVICs were separated to CD34-negative and -positive cells by flow cytometry. We measured real-time PCR, alkaline phosphatase（ALP） activity, Alizarin Red S staining as an index of calcification. Immunohistochemical staining was performed to confirm the distribution of Wnt5a on calcified and normal aortic valves.
Results: HAVICs, especially, CD34-negative cells are highly sensitive to tumor necrosis factor-α（ TNF-α, 30 ng/mL）, and which accelerated the Wnt5a gene expression. Wnt5a antagonist, box5, did not down-regulate HAVIC calcification induced by TNF-α. Further, Wnt5a agonist, foxy5, did not accelerated HAVIC calcification induced by TNF-α. There was no significant difference in the TNF-α-induced acceleration of ALP activity between the cells treated with foxy5 and box5. Furthermore, the proportion of Wnt5a positive cells was no difference between calcified and normal valves.
Conclusions: We confirmed that Wnt5a pathway does not regulate the TNF-α-induced aortic valve calcification in HAVICs obtained from CAVS patients. |
| 書誌情報 |
弘前医学
en : Hirosaki Medical Journal
巻 73,
号 1-4,
p. 20-29,
発行日 2023-03-22
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科 |
| 資源タイプ |
|
|
値 |
Article |
Hirosaki Med J 73-1-4_20 (1.2 MB)
