| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-09-18 |
| タイトル |
|
|
タイトル |
Anti-tumor eff ect of nano-particulated bacillus Calmette-Guerin（BCG）cell wall complex on bladder cancer cell line |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
nano-particulated BCG |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
direct anti-tumor effect |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
bladder cancer cell line |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Mori, Kazuyuki
Yoshikawa, Kazuaki
Saitoh, Hideki
Kudoh, Seiji
Okamoto, Akiko
Imai, Atsushi
Ishimura, Hirofumi
Hatakeyama, Shingo
Hagisawa, Shigeru
Iwabuchi, Ikuya
Yoneyama, Takahiro
Koie, Takuya
Yamato, Takashi
Yokomizo, Hidehiro
Naka, Takashi
Yano, Ikuya
Ohyama, Chikara
|
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Japan BCG Central Laboratory |
| 著者所属 |
|
|
値 |
Japan BCG Central Laboratory |
| 著者所属 |
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|
値 |
Japan BCG Central Laboratory |
| 著者所属 |
|
|
値 |
Department of Urology, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bacillus Calmette-Guerin（ BCG） has been widely accepted as an eff ective treatment for CIS and superfi cial carcinoma of the bladder. However, it has considerable side eff ects and toxicity. We thought if eff ective bacterial cell wall elements as substitutes for live bacteria were identifi ed, useful treatment with lower toxicity while maintaining strong anti-tumor eff ects might be possible. For these reasons we generated a nano-particulated BCG complex, which does not contain live bacteria. Here, we present its direct in vitro anti-tumor eff ect on bladder cancer cell lines. Tokyo 172 BCG sub-strain was disrupted by French press with monitoring the particle distribution by the particle analyzer. After removing the not-disrupted bacteria by centrifuge at 6,800×g, supernatant was centrifuged at 18,000×g. Then the supernatant（ Sup） and the precipitate（ CW） were lyophilyzed to obtain nano-particulated BCG complex. Bladder cancer cell lines, J82 and KK47, were co-cultured with BCG, Sup, CW or Sup+CW（ mix） for 5 days, then viable cell numbers were counted. In J82 cells, when separately added to the culture medium, both CW and Sup reduced cell number to about 70% of control cells. While they were mixed together, they reduced cell number equally compared with BCG; 59.2% （mix） vs 60.2%（ BCG） in J82 cells, 67.3% vs 68.8% in KK47 cells. These preliminary in vitro experiments demonstrated the identical direct anti-tumor eff ect of nano-particulated BCG to that of live BCG. In vivo tumor assays are warranted for clinical application of nano-particulated BCG. |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 59(Suppl.), 2007, p.S162-S166 |
| 書誌情報 |
弘前医学
巻 59,
号 Supplement,
p. S162-S166,
発行日 2007-11-29
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
493.8 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
内科系臨床医学 |
| 出版者 |
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|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_59_S162.pdf (241.8 kB)
