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The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro
http://hdl.handle.net/10129/2233
http://hdl.handle.net/10129/22337d02b4e6-fdae-4316-ab05-50b2de8c62fa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HirosakiMedJ_59_S172.pdf (872.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2009-09-18 | |||||||||||||||||||||
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| タイトル | The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 著者 |
Kusumi, Akinori
× Kusumi, Akinori
× Sakaki, Hirotaka
× Kusumi, Tomomi
× Nakagawa, Hiroshi
× Kubota, Kosei
× Oda, Mitsuo
× Satoh, Hisashi
× Kimura, Hiroto
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| 著者所属 | ||||||||||||||||||||||
| The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine | ||||||||||||||||||||||
| 著者所属 | ||||||||||||||||||||||
| The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine | ||||||||||||||||||||||
| 著者所属 | ||||||||||||||||||||||
| Pathology and Bioscience, National University Corporation, Hirosaki University Graduate School of Medicine | ||||||||||||||||||||||
| 著者所属 | ||||||||||||||||||||||
| The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine | ||||||||||||||||||||||
| 著者所属 | ||||||||||||||||||||||
| The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine | ||||||||||||||||||||||
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| The Department of Oral Anatomy, Showa University School of Dentistry | ||||||||||||||||||||||
| 著者所属 | ||||||||||||||||||||||
| The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine | ||||||||||||||||||||||
| 著者所属 | ||||||||||||||||||||||
| The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine | ||||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | The application of mechanical stress is known to be the unique method of analyses on bone metabolism and remodeling in vitro and in vivo. We have investigated on the effects of the application of cyclic tensile strain （CTS） on the syntheses of bone metabolic factors from the normal human osteoblasts in vitro. In this study, we found that diff erent passage numbers aff ected synthesis of CTS-induced factors from osteoblasts in vitro. Osteoblasts were stimulated with the application of CTS for 3 days, 4 hrs a day. Then, it was found that the application of CTS increased nitric oxide（ NO） production, the expression of cyclooxygenase 2（ Cox-2） mRNA, and synthesis of osteopontin（ OPN） from osteoblasts during serial passage. However, CTS-induced osteoprotegerin（ OPG） synthesis from osteoblasts was shown to change at diff erent passage numbers. In short, increasing at the 3rd passage, CTS-induced OPG synthesis from osteoblasts decreased at the 5th passage. While, though soluble RANKL（ sRANKL） release by the application of CTS decreased at 1st passage and did not change at the 5th passage, the expression of receptor activator of nuclear factor-κB ligand（ RANKL） mRNA increased in osteoblasts at the 5th passage, decreasing in those at the 3rd passage. The increase on OPN synthesis the expression of Cox-2 mRNA, and NO production from osteoblasts by the application of CTS was not aff ected during serial passage. Analyzing on the activity of the p38 mitogen-activated protein kinase（ p38 MAPK） in osteoblasts with or without the application of CTS, the p38 MAPK activity in osteoblasts at the 3rd passage inhibited in compared with that at the 3rd passage. From these results, we should use osteoblasts at the 1st passage when analyzing on CTS-induced OPG synthesis with normal human osteoblasts. It is suggested that the regulation of CTSinduced OPG synthesis might be aff ected by aging of osteoblasts. |
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| 引用 | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | 弘前医学. 59(Suppl.), 2007, p.S172-S187 | |||||||||||||||||||||
| 書誌情報 |
弘前医学 巻 59, 号 Supplement, p. S172-S187, 発行日 2007-11-29 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 0439-1721 | |||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AN00211444 | |||||||||||||||||||||
| フォーマット | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| 日本十進分類法 | ||||||||||||||||||||||
| 主題Scheme | NDC | |||||||||||||||||||||
| 主題 | 491 | |||||||||||||||||||||
| NIIサブジェクト | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | 基礎医学 | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | 弘前大学大学院医学研究科・弘前医学会 | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 値 | Article | |||||||||||||||||||||
