| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-08-13 |
| タイトル |
|
|
タイトル |
＜Symposium I＞A new imaging method of glucose transport in live cells |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
2-NBDG |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
2-NBDLG |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
GLUTs |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Glucose Uptake |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
L-Glucosamine |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Yamada, Katsuya
Yamamoto, Toshihiro
Watanabe, Seiji
Nishiuchi, Yuji
Teshima, Tadashi
Matsuoka, Hideaki
Suga, Sechiko
|
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
12397 |
|
|
姓名 |
ヤマダ, カツヤ |
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
12398 |
|
|
姓名 |
ワタナベ, セイジ |
| 著者(ヨミ) |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
12399 |
|
|
姓名 |
スガ, セチコ |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
12400 |
|
|
姓名 |
山田, 勝也 |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
9443 |
|
|
識別子Scheme |
研究者総覧 |
|
|
識別子URI |
http://hue2.jm.hirosaki-u.ac.jp/html/188_ja.html |
|
|
識別子 |
188 |
|
|
姓名 |
渡邉, 誠ニ |
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
12402 |
|
|
姓名 |
菅, 世智子 |
| 著者所属 |
|
|
値 |
Dept. Physiol., Hirosaki Univ. Grad. Sch. Med. |
| 著者所属 |
|
|
値 |
Saito Res. Center, Peptide Institute |
| 著者所属 |
|
|
値 |
Department of Anatomical Science, Hirosaki Univ. Grad. Sch. Med. |
| 著者所属 |
|
|
値 |
Saito Res. Center, Peptide Institute |
| 著者所属 |
|
|
値 |
Saito Res. Center, Peptide Institute |
| 著者所属 |
|
|
値 |
Department of Biotechnology and Life Science Faculty of Technology, Tokyo University of Agriculture and Technology |
| 著者所属 |
|
|
値 |
Center Res. & Edu. Lifelong Learning., Hirosaki Univ. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Glucose transport activity in mammalian cells has been monitored by radiolabeled tracers such as [14C] 2-deoxy-D-glucose. However, due to their limited spatial and temporal resolution, measuring glucose uptake in single, living cells was diffi cult. We have developed a fl uorescent D-glucose derivative, 2-[N（- 7-nitrobenz-2-oxa-1,3-diazol-4-yl）amino]-2-deoxy-D-glucose （2-NBDG）, that allows a more sensitive measurement of glucose uptake in live cells. Indeed, the uptake of 2-NBDG takes place through glucose transporters （GLUTs） in a concentration-, time-, and temperature-dependent manner. Kinetic analysis revealed that apparent Km values for the uptake were similar to those reported by D-glucose and the nonmetabolizable glucose analogue, 3-O-methyl-D-glucose, found in pancreatic islet cells. This method can be combined with Ca2+ imaging and subsequent immunocytochemical identifi cation of cells. So far, 2-NBDG has been used for monitoring glucose uptake into a wide variety of mammalian cells including astrocytes and neurons. However, it has been pointed out that 2-NBDG method requires very accurate procedures, since the fl uorescence intensity is an arbitrary measure. In addition, temporal changes in health of cells in question should be verifi ed throughout the experiment by an independent way such as using patch clamp. To overcome the diffi culty, we synthesized transporter- unrecognizable glucose analogues as control for 2-NBDG uptake. These include 2-[N（- 7-Nitrobenz-2-oxa-1,3-diazol-4-yl）amino]-2-deoxy-L-glucose [2-NBDLG], the antipode of 2-NBDG. A combined use of transporter-recognizable（ D-isomer） and unrecognizable（ L-isomer） fl uorescent analogues allows us to investigate not only net stereospecifi c transport of glucose in live cells but also ligand-transporter interactions and non-transportermediated glucose movement. |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 61(Suppl.), 2010, p.S23-S25 |
| 書誌情報 |
弘前医学
巻 61,
号 Supplement,
p. S23-S25,
発行日 2010-07-08
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_61_S23.pdf (84.5 kB)
