| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-08-16 |
| タイトル |
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タイトル |
＜Symposium I＞Vascular endothelial growth factor（ VEGF） expression is negatively regulated by basic-helix-loop-helix（ bHLH）transcription factor DEC2 |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
transcription factor |
| キーワード |
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主題Scheme |
Other |
|
主題 |
vascular endothelial growth factor (VEGF) |
| キーワード |
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主題Scheme |
Other |
|
主題 |
hypoxia |
| キーワード |
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主題Scheme |
Other |
|
主題 |
hypoxia-inducible factor-1α(HIF-1α) |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kijima, Hiroshi
Sato, Fuyuki
Bhawal, Ujjal Kumar
Kawamoto, Takeshi
Fujimoto, Katsumi
Imaizumi, Tadaatsu
Imanaka, Tadanobu
Kondo, Jun
Koyanagi, Satoru
Noshiro, Mitsuhide
Yoshida, Hidemi
Kato, Yukio
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| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
12420 |
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姓名 |
キジマ, ヒロシ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
12421 |
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姓名 |
サトウ, フユキ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20588 |
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姓名 |
ウジャール, クマール バイアル |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20589 |
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姓名 |
カワモト, タケシ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20590 |
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姓名 |
フジモト, カツミ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
11451 |
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姓名 |
イマイズミ, タダアツ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20591 |
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姓名 |
イマナカ, タダノブ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
12423 |
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姓名 |
コンドウ, ジュン |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20592 |
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姓名 |
コヤナギ, サトル |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20593 |
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姓名 |
ノシロ, ミツヒデ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
11452 |
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姓名 |
ヨシダ, ヒデミ |
| 著者(ヨミ) |
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識別子Scheme |
WEKO |
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識別子 |
20594 |
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姓名 |
カトウ, ユキオ |
| 著者所属 |
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Department of Pathology, Hirosaki University School of Medicine |
| 著者所属 |
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Department of Pathology, Hirosaki University School of Medicine |
| 著者所属 |
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Department of Oral Maxillofacial Diagnostic Science, Division of Pathology & High-Tech Research Center, Kanagawa Dental College |
| 著者所属 |
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Department of Oral Maxillofacial Diagnostic Science, Division of Pathology & High-Tech Research Center, Kanagawa Dental College |
| 著者所属 |
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Department of Oral Maxillofacial Diagnostic Science, Division of Pathology & High-Tech Research Center, Kanagawa Dental College |
| 著者所属 |
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Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine |
| 著者所属 |
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Department of the Biology and Medicine of the Stem Cell, Hiroshima University Graduate School of Medical Science |
| 著者所属 |
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Department of Pathology, Hirosaki University School of Medicine |
| 著者所属 |
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Department of Medico-Pharmaceutical Sciences, Kyushu University |
| 著者所属 |
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Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences |
| 著者所属 |
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Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine |
| 著者所属 |
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Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The circadian rhythms in mammals are regulated by a pacemaker located in the suprachiasmatic nucleus of the hypothalamus. Five clock-gene families, i.e. Clock, Bmal, Per, Cry and Dec, have been found to be involved in a transcription-translation feedback loop that generates the circadian rhythm at the intracellular level. In this study, we examined functional analysis of the Dec gene. DEC1 and DEC2 are basic-helix-loop-helix （bHLH） transcription factors, involved in cellular diff erentiation, responses to hypoxia, and circadian rhythms. We recently showed that the expression of DEC1 and DEC2 was upregulated by hypoxia, however, the functions of these two factors under hypoxic conditions have not been elucidated in detail. It is well established that the expression of vascular endothelial growth factor （VEGF） is upregulated by hypoxia, and the expression of VEGF in response to hypoxia depends on transcriptional activation by a heterodimer comprising hypoxia-inducible factor 1 α （HIF-1α） and arylhydrocarbon receptor nuclear translocator 1 （ARNT1）. In the present study, we showed that DEC2, but not DEC1, suppressed VEGF gene expression under hypoxic conditions. DEC2 protein was co-immunoprecipitated with HIF-1α but not with ARNT1. The binding of HIF-1α to the hypoxia response element（ HRE） in the VEGF promoter was decreased by DEC2 overexpression, and increased by DEC2 knockdown. We also showed that the circadian expression of VEGF showed a reciprocal pattern to that of DEC2 in cartilage. DEC2 had a circadian oscillation in implanted Sarcoma 180 cells. We conclude that DEC2 negatively regulates VEGF expression and plays an important role in the pathological conditions in which VEGF is involved. |
| 引用 |
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内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 61(Suppl.), 2010, p.S43-S52 |
| 書誌情報 |
弘前医学
巻 61,
号 Supplement,
p. S43-S52,
発行日 2010-07-08
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0439-1721 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
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主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
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値 |
Article |
HirosakiMedJ_61_S43.pdf (252.6 kB)
