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Simultaneous Measurement of Adenosine 3’,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate in Biological Samples
http://hdl.handle.net/10129/6148
http://hdl.handle.net/10129/6148490a42ba-4ffb-4609-af2b-50d6708ee6ce
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HirosakiMedJ_68(1)_52.pdf (276.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2017-10-23 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Simultaneous Measurement of Adenosine 3’,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate in Biological Samples | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | adenosine 3’,5’-cyclic monophosphate | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | guanosine 3’,5’-cyclic monophosphate | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Sep-Pak amino propyl cartridge | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | enzymatic cycling | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | aortic smooth muscle cells | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
Seya, Kazuhiko
× Seya, Kazuhiko
× Motomura, Shigeru
× Imaizumi, Tadaatsu
× Furukawa, Ken-Ichi
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| 著者所属 | ||||||||||||||
| Department of Pharmacology | ||||||||||||||
| 著者所属 | ||||||||||||||
| Department of Pharmacology | ||||||||||||||
| 著者所属 | ||||||||||||||
| Department of Vascular Biology, Hirosaki University Graduate School of Medicine | ||||||||||||||
| 著者所属 | ||||||||||||||
| Department of Pharmacology | ||||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Two sensitive enzymatic fluorometric assays have been developed for adenosine 3’,5’-cyclic monophosphate （cAMP） by Sugiyama et al. （Anal. Biochem. 1990） and for guanosine 3’,5’-cyclic monophosphate （cGMP） by Seya et al. （Anal. Biochem. 1998）. However, to make a new simultaneous comparison of two cyclic nucleotides, distinct measurement methods cause less reliability and longer measurement time. To overcome these problems, we developed a simultaneous measurement method for them. All adenosine nucleotides and GMP were enzymatically degraded using alkaline phosphatase and apyrase. The remaining GDP was converted to GTP by creatine kinase. Cyclic GMP and cAMP, absorbed into a Sep-Pak amino propyl cartridge, were eluted to separate from GTP, and then were simultaneously quantified using improved enzymatic fluorometric assay. The detection limits for cAMP and cGMP were 1 and 5 fmol, respectively. The total measurement time was about 10 h. Using this method, the basal cAMP and cGMP levels in rat aortic smooth muscle cells were confirmed to 4.1 and 0.042 pmol/mg protein, respectively. An adrenergic agonist, isoproterenol （1 μM） increased cAMP approximately 6 fold, while nitric oxide donor, S-nitroso-N-acetylpenicillamine （100 μM） increased cGMP approximately 230 fold. These results suggest that this simultaneous measurement of cGMP and cAMP can provide a more convenient assessment of them in biological samples. |
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| 引用 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | 弘前医学. 68, p.52-61. 2017 | |||||||||||||
| 書誌情報 |
弘前医学 巻 68, 号 1, p. 52-61, 発行日 2017-10-05 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 0439-1721 | |||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AN00211444 | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| NIIサブジェクト | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | 医学 | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | 弘前大学大学院医学研究科・弘前医学会 | |||||||||||||
| 言語 | ja | |||||||||||||
| 資源タイプ | ||||||||||||||
| 値 | Article | |||||||||||||
