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Immunohistochemical localization of mesenchymal stem cells in ossified human spinal ligaments
http://hdl.handle.net/10129/5113
http://hdl.handle.net/10129/5113f68615e8-9340-4eb7-8310-cd9ed4a68789
| 名前 / ファイル | ライセンス | アクション |
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内容要旨 (132.2 kB)
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本文 (605.6 kB)
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審査要旨 (98.4 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-12-20 | |||||
| タイトル | ||||||
| タイトル | Immunohistochemical localization of mesenchymal stem cells in ossified human spinal ligaments | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Spinal ligament ossification | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mesenchymal stem cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Immunohistochemistry | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Localization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Neovascularization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chondrocytes | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
Chin, Shunfu
× Chin, Shunfu× Furukawa, Ken-Ichi× Ono, Atsushi× Asari, Toru× Harada, Yoshifumi× Wada, Kanichiro× Tanaka, Toshihiro× Inaba, Wataru× Mizukami, Hiroki× Motomura, Shigeru× Yagihashi, Soroku× Ishibashi, Yasuyuki |
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| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15713 | |||||
| 姓名 | チン, シュンフ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 12001 | |||||
| 姓名 | フルカワ, ケンイチ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15715 | |||||
| 姓名 | オノ, アツシ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15716 | |||||
| 姓名 | アサリ, トオル | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15525 | |||||
| 姓名 | ハラダ, ヨシフミ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15718 | |||||
| 姓名 | ワダ, ケンイチロウ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15093 | |||||
| 姓名 | タナカ, トシヒロ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15720 | |||||
| 姓名 | イナバ, ワタル | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 14200 | |||||
| 姓名 | ミズカミ, ヒロキ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 12114 | |||||
| 姓名 | モトムラ, シゲル | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15723 | |||||
| 姓名 | ヤギハシ, ソウロク | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 10707 | |||||
| 姓名 | イシバシ, ヤスユキ | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 15725 | |||||
| 姓名 | 陳, 俊輔 | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mesenchymal stem cells (MSCs) have been isolated from various tissues and used for elucidating the pathogenesis of numerous diseases. In our previous in vitro study, we showed the existence of MSCs in human spinal ligaments and hypothesized that these MSCs contributed to the pathogenesis of ossification of spinal ligaments. The purpose of this study was to use immunohistochemical techniques to analyze the localization of MSCs in ossified human spinal ligaments in situ. Ossified (OLF) or non-ossified ligamentum flavum (non-OLF) samples from the thoracic vertebra were obtained from patients who had undergone posterior spinal surgery. Serial sections were prepared from paraffin-embedded samples, and double immunofluorescence staining was performed using antibodies against markers for MSCs (CD73, CD90 and CD105), endothelial cells (CD31), pericytes (α-smooth muscle actin), and chondrocytes (S100). Immunolocalization of MSCs was observed in the perivascular area and collagenous matrix in spinal ligaments. Markers for MSCs and pericytes were co-expressed in the perivascular area. Compared with non-OLF, OLF had a large amount of neovascularization in the fragmented ligament matrix, and a high accumulation of MSCs around blood vessels. The prevalence of MSCs in OLF within collagenous matrix was significantly higher than that in non-OLF. Chondrocytes near the ossification front in OLF also presented expression of MSC markers. MSCs may contribute to the ectopic ossification process of OLF through endochondral ossification. | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Biochemical and biophysical research communications, 436(4), 2013, p.698-704 | |||||
| 書誌情報 |
Biochemical and biophysical research communications 巻 436, 号 4, p. 698-704, 発行日 2013-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-291X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00564395 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.bbrc.2013.06.019 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 494.66 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | ETD | |||||
| NIIサブジェクト | ||||||
| 主題Scheme | Other | |||||
| 主題 | 外科系臨床医学 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 資源タイプ | ||||||
| 値 | Thesis | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 11101 | |||||
| 学位授与機関名 | 弘前大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2013-09-30 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲1811 | |||||
