| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-03-07 |
| タイトル |
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タイトル |
Engraftment and morphological development of vascularized human iPS cell-derived 3D-cardiomyocyte tissue after xenotransplantation |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Narita, Hirokazu
Shima, Fumiaki
Yokoyama, Junya
Miyagawa, Shigeru
Tsukamoto, Yoshinari
Takamura, Yasushi
Hiura, Ayami
Fukumoto, Ken
Chiba, Tomohiro
Watanabe, Seiji
Sawa, Yoshiki
Akashi, Mitsuru
Shimoda, Hiroshi
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| 著者所属 |
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Hirosaki Univ, Dept Anat Sci, Grad Sch Med |
| 著者所属 |
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Osaka Univ, Dept Frontier Biosci, Grad Sch Frontier Biosci |
| 著者所属 |
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Osaka Univ, Dept Cardiovasc Surg, Grad Sch Med |
| 著者所属 |
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Osaka Univ, Dept Cardiovasc Surg, Grad Sch Med |
| 著者所属 |
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Osaka Univ, Dept Frontier Biosci, Grad Sch Frontier Biosci |
| 著者所属 |
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Osaka Univ, Dept Frontier Biosci, Grad Sch Frontier Biosci |
| 著者所属 |
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Osaka Univ, Dept Frontier Biosci, Grad Sch Frontier Biosci |
| 著者所属 |
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Kyowa Hakko Bio Co LTD |
| 著者所属 |
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Hirosaki Univ, Dept Anat Sci, Grad Sch Med |
| 著者所属 |
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Hirosaki Univ, Dept Anat Sci, Grad Sch Med |
| 著者所属 |
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Osaka Univ, Dept Cardiovasc Surg, Grad Sch Med |
| 著者所属 |
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Osaka Univ, Dept Frontier Biosci, Grad Sch Frontier Biosci |
| 著者所属 |
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Hirosaki Univ, Dept Anat Sci, Grad Sch Med |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
One of the major challenges in cell-based cardiac regenerative medicine is the in vitro construction of three-dimensional (3D) tissues consisting of induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) and a blood vascular network supplying nutrients and oxygen throughout the tissue after implantation. We have successfully built a vascularized iPSC-CM 3D-tissue using our validated cell manipulation technique. In order to evaluate an availability of the 3D-tissue as a biomaterial, functional morphology of the tissues was examined by light and transmission electron microscopy through their implantation into the rat infarcted heart. Before implantation, the tissues showed distinctive myofibrils within iPSC-CMs and capillary-like endothelial tubes, but their profiles were still like immature. In contrast, engraftment of the tissues to the rat heart led the iPSC-CMs and endothelial tubes into organization of cell organelles and junctional apparatuses and prompt development of capillary network harboring host blood supply, respectively. A number of capillaries in the implanted tissues were derived from host vascular bed, whereas the others were likely to be composed by fusion of host and implanted endothelial cells. Thus, our vascularized iPSC-CM 3D-tissues may be a useful regenerative paradigm which will require additional expanded and long-term studies. |
| 書誌情報 |
SCIENTIFIC REPORTS
巻 7,
p. 13708,
発行日 2017-10-20
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2045-2322 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41598-017-14053-0 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 資源タイプ |
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値 |
Article |
s41598-017-14053-0 (3.3 MB)
