| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-03-08 |
| タイトル |
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タイトル |
Clinical implications of serum N-glycan profiling as a diagnostic and prognostic biomarker in germ-cell tumors |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Narita, Takuma
Hatakeyama, Shingo
Yoneyama, Tohru
Narita, Shintaro
Yamashita, Shinichi
Mitsuzuka, Koji
Sakurai, Toshihiko
Kawamura, Sadafumi
Tochigi, Tatsuo
Takahashi, Ippei
Nakaji, Shigeyuki
Tobisawa, Yuki
Yamamoto, Hayato
Koie, Yoichi
Tsuchiya, Norihiko
Habuchi, Tomonori
Arai, Yoichi
Ohyama, Chikara
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| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Adv Transplant & Regenerat Med |
| 著者所属 |
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Akita Univ, Grad Sch Med, Dept Urol |
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Tohoku Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Tohoku Univ, Grad Sch Med, Dept Urol |
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Yamagata Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Miyagi Canc Ctr, Dept Urol |
| 著者所属 |
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Miyagi Canc Ctr, Dept Urol |
| 著者所属 |
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Hirosaki Univ, Sch Med, Dept Social Med |
| 著者所属 |
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Hirosaki Univ, Sch Med, Dept Social Med |
| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Yamagata Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Akita Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Tohoku Univ, Grad Sch Med, Dept Urol |
| 著者所属 |
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Hirosaki Univ, Grad Sch Med, Dept Urol |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Serum biomarker monitoring is essential for management of germ-cell tumors (GCT). However, not all GCT are positive for conventional tumor markers. We examined whether serum N-glycan-based biomarkers can be applied for detection and prognosis in patients with GCT. We performed a comprehensive N-glycan structural analysis of sera from 54 untreated GCT patients and 103 age-adjusted healthy volunteers using glycoblotting methods and mass spectrometry. Candidate N-glycans were selected from those with the highest association; cutoff concentration values were established, and an N-glycan score was created based on the number of positive N-glycans present. The validity of this score for diagnosis and prognosis was analyzed using a receiver operating characteristic (ROC) curve. We identified five candidate N-glycans significantly associated with GCT patients. The accuracy of the N-glycan score for GCT was significant with an area-under-the-curve (AUC) value of 0.87. Diagnostically, the N-glycan score detected 10 of 12 (83%) patients with negative conventional tumor markers. Prognostically, the N-glycan score comprised four candidate N-glycans. The predictive value of the prognostic N-glycan score was significant, with an AUC value of 0.89. A high value prognostic N-glycan score was significantly associated with poor prognosis. Finally, to identify a potential carrier protein, immunoglobulin (Ig) fractions of sera were subjected to N-glycan analysis and compared to whole sera. Candidate N-glycans in Ig-fractions were significantly decreased; therefore, the carrier protein for candidate N-glycans is likely not an immunoglobulin. In summary, our newly developed N-glycan score seems to be a practical diagnostic and prognostic method for GCT. |
| 書誌情報 |
CANCER MEDICINE
巻 6,
号 4,
p. 739-748,
発行日 2017-04
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2045-7634 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1002/cam4.1035 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 資源タイプ |
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値 |
Article |
cam4.1035 (425.6 kB)
