| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-04-09 |
| タイトル |
|
|
タイトル |
Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
biliary atresia |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
retinoic acid-inducible gene-I |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
CXCL10 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
HuCCT1 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
poly（I:C）. |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kimura, Toshiro
Imaizumi, Tadaatsu
Yoshida, Tatsuya
Shimada, Taku
Hayakari, Ryo
Kawaguchi, Shogo
Yoshida, Hidemi
Kobayashi, Tamotsu
Hirabayashi, Takeshi
Mizukami, Hiroki
Kijima, Hiroshi
Hakamada, Kenichi
|
| 著者所属 |
|
|
値 |
Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Gastroenterology and Hematology,Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital |
| 著者所属 |
|
|
値 |
Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital |
| 著者所属 |
|
|
値 |
Department of Pathology and Molecular Medicine,Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Purpose: Retinoic acid-inducible gene-I （RIG-I） is a member of cytoplasmic viral sensors which plays an important role in inflammation of biliary epithelial cells（ BECs）. The aim of this study is to examine if RIG-I and C-X-C motif chemokine 10（ CXCL10） are involved in the etiology of human biliary atresia（ BA）.
Methods: Immunohistochemical study was performed on surgically resected tissues obtained（ June 1994 to March 2016）from 30 infants with BA and non-inflamed hepatic tissues from 7 infants with hepatoblastoma. A semiquantitative scoring system was designed to evaluate the staining with an antibodies to the RIG-I and CXCL10. The expression of RIG-I and CXCL10 in HuCCT1 cholangiocarcinoma cell line were studied by western blotting, ELISA and RT-PCR analyses.
Results: Intense immunoreactivity for RIG-I and CXCL10 was detected in BECs in tissues resected from BA patients.The expression of RIG-I and CXCL10 in the hilar tissue was significantly stronger than in the hepatic tissue.Transfection of HuCCT1 cells with poly（I:C）, a synthetic analog of viral dsRNA, induced the expression of RIG-I, and knockdown of RIG-I inhibited the induction of CXCL10 in HuCCT1 cells transfected with poly（I:C）.
Conclusion: These results suggest that RIG-I-CXCL10 cascade may be involved in the etiology of human BA. |
| 書誌情報 |
弘前医学
巻 69,
号 1-4,
p. 86-94,
発行日 2019-03-15
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
HirosakiMedJ_69(1-4)_86.pdf (489.6 kB)
