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Hyaluronan synthesis inhibitor 4-methylumbelliferone affects extracellular matrix components in cultured human skin fibroblasts
http://hdl.handle.net/10129/00006844
http://hdl.handle.net/10129/00006844c3bf81f5-aae8-4056-81ad-1fb93010dada
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HirosakiMedJ_70(1)_1 (315.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-22 | |||||
| タイトル | ||||||
| タイトル | Hyaluronan synthesis inhibitor 4-methylumbelliferone affects extracellular matrix components in cultured human skin fibroblasts | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hyaluronan | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hyaluronan synthesis inhibitor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 4-methylumbelliferone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | extracellular matrix | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Endo, Yasufumi
× Endo, Yasufumi× Takahashi, Gen× Kakizaki, Ikuko× Endo, Masahiko |
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| 著者所属 | ||||||
| 値 | Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Anatomical Science, Hirosaki University Graduate School of Health Sciences | |||||
| 著者所属 | ||||||
| 値 | Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Abstract: Hyaluronan （HA） is a ubiquitous glycosaminoglycan comprising N-acetylglucosamine and glucuronic acid and is involved in matrix formation and cell maintenance. 4-Methylumbelliferone （MU） exhibits anti-tumor activities by inhibiting HA synthesis. In this study, we aimed to elucidate the association between HA and numerous matrix molecules. Materials and Methods: We created HA-knock down extracellular matrix （ECM） by culturing human skin fibroblasts（ HSF） in the presence of MU（ 0.5 mM） for 3 days after confluence. Following culture in the presence or absence of MU, the cell layer and culture medium were collected and ECM molecules were measured using enzymelinked immunosolvent assay, and the［ 35S］-sulfate uptake method. Results: Proteoglycans and type I collagen were released into the medium as HA decreased. However, type collagen, but not proteoglycans, was immediately replenished. Fibronectin and CD44 in the cell layer decreased, but were not replenished, as HA decreased. On contrary, laminin, cadherin, and vitronectin were not affected by HA decrease. Conclusions: HA knockdown by MU treatment elucidated the dynamic interactions between HA and ECM components. Our findings suggest that HA interacts with other ECM components through various ways, including |
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| 書誌情報 |
弘前医学 巻 70, 号 1, p. 1-12, 発行日 2019-11-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0439-1721 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00211444 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 弘前大学大学院医学研究科・弘前医学会 | |||||
