| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-04-21 |
| タイトル |
|
|
タイトル |
Effect of bisphosphonate alendronate on ERK-dependent growth in human gingival fibroblasts |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
MRONJ |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
alendronate |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Bisphosphonate |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
gingival fibroblasts |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
MAP kinas |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Akiyama, Natsumi
Kubota, Kosei
Komatsu, Shotaro
Itoh, Ryohei
Chang, Hao Chi
Yamazaki, Shunya
Imaizumi, Tadaatsu
Matsumiya, Tomoh
Kobayashi, Wataru
|
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine / Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences |
| 著者所属 |
|
|
値 |
Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Objective: Medication-related osteonecrosis of the jaw （MRONJ） is a complication of bisphosphonate （BP） therapy. The pathophysiology of MRONJ remains unclear. Extensive studies have been performed to examine the effect of BPs on bone metabolism. Breakdown of mucosa is a factor classified into five stages in MRONJ, indicating an important role of oral soft tissue in the pathogenesis of MRONJ.
Methods: We investigated the effect of alendronate, one of the most commonly used BPs worldwide, on the proliferation of human gingival fibroblasts by MTT assay, western blotting, and wound healing assay.
Results: We observed time- and concentration-dependent inhibition of fibroblast proliferation by alendronate. Wound healing assays also showed that alendronate prolonged wound healing in a concentration-dependent manner. MAP kinase signaling pathway exhibits a cardinal role in cell proliferation; however, little is known about whether BPs affect classical MAP kinase ERK in fibroblasts. We found that U0126, a selective inhibitor of MAP kinase kinase （MEK1/2）, inhibited fibroblast proliferation similarly to alendronate. Alendronate was also found to inactivate ERK1/2, both of which are downstream molecules of MEK1/2.
Conclusion: our findings indicated that alendronate inhibits the proliferation of human gingival fibroblasts via inactivation of ERK1/2. |
| 書誌情報 |
弘前医学
en : Hirosaki Medical Journal
巻 73,
号 1-4,
p. 30-39,
発行日 2023-03-22
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科 |
| 資源タイプ |
|
|
値 |
Article |
Hirosaki Med J 73-1-4_30 (413.8 kB)
