| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-05-25 |
| タイトル |
|
|
タイトル |
Role of Protein Kinase C in Acetylcholine-Induced Ca2+ Influx under Enhanced Phospholipase C-δ1 |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Coronary spasm |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Protein kinase C |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Phospholipase C-δ1 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Calcium influx |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Calcium influx |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Acetylcholine |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Murakami, Kazuo
Osanai, Tomohiro
Tanaka, Makoto
Kinjo, Takahiko
Tanno, Tomohiro
Narita, Ikuyo
Kawai, Misato
Tomita, Hirofumi
Okumura, Ken
|
| 著者所属 |
|
|
|
Department of Cardiology, Hirosaki University Graduate School of Medicine |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Background: We reported that enhanced phospholipase C （PLC）-δ1, which was detected in patients with coronary spasm, caused coronary spasm in mice. We investigated the role of protein kinase C（ PKC） in acetylcholine （ACh）-induced Ca2+ influx under enhanced PLC-δ1 using human embryonic kidney（ HEK）-293 cells. Methods and Results: Intracellular free Ca2+ concentration （[Ca2+]i） was measured by fura-2, and Ca2+ influx was evaluated by the increase in [Ca2+]i after addition of extracellular Ca2+. ACh-induced Ca2+ influx（ nM） in HEK-293 cells was 21±2 in control HEK-293 cells, 52±6 in the cells with PLC-δ1 overexpression, and 75±9 in those with PLC-δ1 overexpression and PKC down-regulation （all p<0.05 among 3 groups）. Ca2+ influx under treatment with nifedipine at 10-5M was 2.9±0.1 times higher in the cells with PLC-δ1 overexpression and 5.6±0.2 times higher in those with PLC-δ1 overexpression and PKC down-regulation compared with the control cells（ all p<0.05 among 3 groups）. Conclusions: ACh-induced Ca2+ influx was enhanced by PLC-δ1 overexpression, but was attenuated by PKC activation. PKC plays an important role in Ca2+ influx under enhanced PLC-δ1 in a negative feedback fashion. |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
弘前医学. 66, 2015, p.22-27 |
| 書誌情報 |
弘前医学
巻 66,
号 1,
p. 22-27,
発行日 2015-04-06
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0439-1721 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00211444 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| NIIサブジェクト |
|
|
主題Scheme |
Other |
|
主題 |
医学 |
| 出版者 |
|
|
出版者 |
弘前大学大学院医学研究科・弘前医学会 |
| 資源タイプ |
|
|
値 |
Article |
HirosakiMedJ_66(1)_22.pdf (2.5 MB)
